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Cell pellets were resuspended in reducing lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 10 mM DTT, 0.01 mg ml 1 DNase I) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator

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2.2.1 Salkowski colorimetric method The original protocol of the Salkowski assay reported by Gordon and Weber was adapted for a 96-well plate format ( 3 , 97% reagent grade, and 34.3% perchloric acid, ACS grade)

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