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Although the substance was discovered in 1905, its crucial role in metabolism was not elucidated until 1955, and its deficiency was not described until 1972 (Bhattacharyya 1995

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[39] The fatty-diacid section (eicosanedioic acid) is linked via a glutamic acid and two (2-(2-aminoethoxy)ethoxy)acetic acid units to the side chain of the lysine residue

Samples were then acidified by adding 12% phosphoric acid (1:10 v/v acid to sample)