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[0087] 34.8 g of crude Semaglutide was weighed, dissolved in 1500 mL of a mixed solvent of 25% acetonitrile and 75% water, and purified using Waters 2545 RP-HPLC system with a wavelength of 218 nm, a chromatographic column of 50 x 250 mm reverse phase C18 column, a column temperature of 45C, and NaClO4 and acetonitrile as a mobile phase system
Resin cleavage (peptides) The peptidyl resin was washed with DCM (3 x 15 mL) after synthesis
Solvable Triggers Often Cluster Clinical trials document the scale of these side effects