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The complex was then concentrated using an Amicon Ultra Centrifugal Filter (MWCO, 100 kDa) and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column (GE Healthcare) that was pre-equilibrated with 20mM HEPES pH 7.4, 100mM NaCl, 2mM MgCl 2 , 1M peptide, 0.01% (w/v) LMNG and 0.0006% (w/v) CHS to separate complex from contaminants

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This discomfort usually occurs because the medication slows down gastric emptying, which means food stays in your stomach longer than usual

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Your body stores vitamin B-12 in your muscles and organs, which helps keep nutrient levels up