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Before Your Treatment Your doctor will assess if Botox is the right option for you by: Reviewing your medical history Identify if your migraines are chronic or episodic Checking all the medications you have tried Check your neck and head muscles After Your Treatment These are the basic aftercare you should do: Avoid intense exercise for 24 hours Do not rub the injection sites Stay upright for the next 4 hours Hydrate properly Take pain medicines if needed

[5] Kwon DR, Park GY

doi: 10.3389/fphys.2026.1779008 Received 31 December 2025 Revised 17 February 2026 Accepted 19 February 2026 Published 09 March 2026 Volume 17 - 2026 Edited by Mohd Yaseen Malik, University of Oxford, United Kingdom Reviewed by Jerzy Beltowski, Medical University of Lublin, Poland Gabriel Komla Adzika, University of Pennsylvania, United States Updates Copyright 2026 Pena, Del Ro and Flores

HOW IT WORKS

For best results use at a .5% to 2% concentration, keep in mind 2% is the maximum suggested concentration level for this product

Solvent A (0.1% formic acid in water) and solvent B (acetonitrile) were used as mobile phases