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Its here where one of the small, but subtle improvements have been made

2015 Nov;30(13):1831-7

MD, Mediterranean diet

While there are established pharmacological guidelines for the prevention of migraines (2, 3), a significant number of patients still experience unmet needs, with many not benefiting from or failing to respond to preventative treatments (4, 5)

The secondary antibodies used are as follows: Goat Anti-Mouse IgG H&L (Alexa Fluor 488) (Abcam, ab150113), Goat Anti-Mouse IgG H&L (Alexa Fluor 647) (Abcam, ab150115), Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (Abcam, ab150077), Donkey Anti-Rabbit IgG H&L (Alexa Fluor 647) (Abcam, ab150075)

They were then spun down, removed of their supernatant, and resuspended and washed with several changes of PBS, then washed over night in 5 mL PBS