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Determination of ATP levels ATP was measured using the luciferase-based ATP determination kit (Thermo Fisher, USA

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Furthermore, while in 2D/unconfined environments breast cancer cells maintain a mesenchymal migration phenotype characterized by actin-rich protrusions and adhesion to the substrate [7], in confinement, cells switch to an amoeboid/bleb-based migration mode [152, 164, 165]

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