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[PMC free article: PMC1734378] [PubMed: 10874630] 36

For cell samples, BA and hepatocytes were lysed in lysis buffer (10 mM Tris pH 7.5, 5 mM EDTA, 50 mM HCl, 30 M sodium pyrophosphate, 50 mM NaF, 100 mM sodium o-vanadate, 1% Triton X-100, 1 mM PMSF and 10 g/mL protease inhibitors), pH 7.47.6

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Reconstitution Quick Reference The ratios below reflect community-documented reconstitution protocols, not prescribed instructions

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