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These 496 metabolites were classified as 268 glycerophospholipids, 191 glycerolipids, 36 sphingolipids and one fatty acyl lipid (Supplementary Figure 4B)

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Thus, in our experiments in a murine model of kidney fibrosis, TMAO or choline diet supplementation resulted in hyperactivation of ERK1/2, mTORC1 and SMAD3 pro-fibrotic signaling 46,50 (Supplementary Fig