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For stress analyses in Synechocystis , mid-exponential phase cultures (OD 580 nm = 0.3 to 0.8) were adjusted to OD 580 = 0.02 (5.10 5 cells mL 1 ) for growth analysis or OD 580 = 0.1 (2.5 10 6 cells mL 1 ) for survival analysis and subsequently incubated for various durations in liquid or on solid media containing the indicated noxious agents prior to measuring OD 580 or photographing the flasks culture or plates

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Mitochondria were treated with pyruvate/malate/NADH followed sequentially with rotenone/antimycin A, TMPD/ascorbic acid, and azide (n = 6)

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