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DIV12 the neurons were fixed (4% paraformaldehyde, 3% sucrose, 60 mM PIPES, 25 mM HEPES, 5 mM EGTA, 1 mM MgCl 2 , pH 7.4) for 20 min at room temperature and mounted

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However, the absolute concentrations of metabolites could not be determined in an untargeted metabolomics approach

Related Substances Chromatography should assess deletion sequences, incomplete coupling products, oxidation and hydrolytic degradation