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It has been described for roGFP1-iL that the insertion C147CL and the substitution H148S lower the thermodynamic stability of the inter-strand disulfide C147C204 resulting in a less negative E of the sensor (Lohman and Remington, E roGFP2-iL was determined through titration with LA/DHLA

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As it focuses on fat metabolism, it doesnt alter hormone levels compared to others

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