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Samples were boiled for 5 min at 95 C with loading buffer (10% 2-mercaptoethanol, 4%SDS, 20%glycerol, 0.125M Tris HCl, and 0.004% bromophenol blue PH6.8)

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Obstructive sleep apnea (OSA) syndrome is a sleep disorder strongly associated with both the load-bearing and metabolic and inflammatory implications of obesity, leading to increased CV risk and reduced quality of life 101

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