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A 35 Ultra Semi diamond knife (Diatome) mounted on a UC7 ultramicrotome (Leica) was used to cut alternating series of sections onto formvar-coated copper mesh grids at two different thicknesses: (i) 60 nm-thick sections were visually inspected to assess ultrastructural preservation and synapse density under an 80kV LEO 912 transmission electron microscope (Zeiss), and (ii) 250 nm-thick sections were coated on both surfaces with Protein A-conjugated 10 nm gold fiducial particles (Cell Microscopy Core Products, University Medical Center Utrecht, The Netherlands) in preparation for transmission electron tomography

Es hilft dabei, die schdlichen Auswirkungen von reaktiven Sauerstoffspezies (ROS) auf die Haut zu minimieren

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For future preparations, refrigerate within 10 minutes of reconstitutionset a timer if necessary

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