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doi: 10.1126/science.1214914 232 HepworthMRMonticelliLAFungTCZieglerCGGrunbergSSinhaRet al

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After removing debris and epithelial cells using 100 and 40 m cell strainers, respectively, ESCs were resuspended in DMEM/F12 containing 10% foetal bovine serum (FBS) (Gibco, New York, USA) and 1% penicillinstreptomycin (Gibco, New York, USA) and cultured in 5% CO 2 at 37 C

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