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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SCF

While the intense basal level of infiltrated neutrophils and the establishment of bacterial infections are primary contributors to the generation of ROS in CF, the underlying cellular dysfunction provides additional, intrinsic sources of oxidative stress

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The primary functionally related group of genes differentially expressed across subclusters was related to cell identity and maturity

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