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H 2 S production and the effect of garlic on hypertension A second vascular gaseous signal transmitter is H 2 S.93 H 2 S exists in micromolar concentrations in various mammalian tissues, including the brain, nervous system, vascular smooth muscle cells, and in the heart.93 Endogenous H 2 S production is primarily the result of two enzymes: cystathionine--synthase (CBS) and cystathionine--lyase (CSE), whereby the nonessential amino acid cysteine is metabolized by desulfuration, releasing sulfur in a reduced oxidation state and generating H 2 S