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Subsequently, the chromogen reacts with the thiol group of GSH to produce a colored compound that absorbs at 405 nm

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A p-value of less than 0.05 was deemed statistically significant

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Dark under-eye circles can be caused by various factors, including genetics, thinning skin due to aging, lack of sleep, dehydration, allergies, pigmentation irregularities, and lifestyle habits like smoking or excessive alcohol consumption