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Discussion The main finding in the present study was an increase in peripheral levels of both proBDNF and BDNF in CUD patients who were abstinent from cocaine for a minimum of 2 days before testing compared with healthy individuals

A senescence -galactosidase assay was conducted using the senescence -galactosidase staining kit (Cell Signaling Technology, 9860) according to the manufacturers instructions, and then stained cells were imaged using a ZEISS Primovert inverted microscope (Carl Zeiss Microscopy)

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