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The obtained desalted supernatant (25 l) was incubated with 125 l of 50 mM K-phosphate buffer (pH 7.5), 25 l 0.1 M aminotriazole, 25 l of 2.5 mM GSH and 2.5 U of GR (Sigma G3664) for 10 min at 30 C

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Identification of G protein-coupled receptor genes from the human genome sequence

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