While its unlikely that your animals can overdose on B12, a blood test or other diagnostic tests used by veterinarians can help rule out other issues that may cause symptoms instead
Vitamin B12 injections offer a range of health benefits and can be particularly advantageous for individuals with B12 deficiency, pernicious anemia, or conditions that impair B12 absorption, such as gastrointestinal disorders (e.g., Crohns disease or celiac disease)
Removal or destruction of the stomach: Vitamin B 12 deficiency can develop in people who have had surgery to remove part or all of the stomach
Always match your format to your specific experimental goals
This primarily stems from internal differences, like the amount of intrinsic factor released in the stomach or genetic defects (much like an MTHFR mutation)
They indicate your body is making antibodies that will protect you later, without you having to experience the illness itself. Some reactions are less frequent but still normal as your body responds to the vaccine