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HEK293T cells were transfected with 1 g lentiCRISPRv2 containing the sgRNA insert, 0.67 g psPAX2 (Addgene, 12260) and 0.33 g pVSVg (Addgene, 8454) using 6 g polyethylenimine (Sigma-Aldrich, 919012) in Opti-MEM (Thermo Fisher, 31985062)

the center grant P50AA011999 Southern California Research Center for ALPD and Cirrhosis funded by NIAAA/NIH

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Building Glutathione from the Inside Out Another way to increase glutathione is to provide the body with the raw materials and cofactors to make and maintain it