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This identified the reduction in butyrate-producing taxa as a signature of gut microbiome shifts in type 2 diabetes

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Cell pellets (~2030 l of packed cell pellet) were re-suspended in 1 ml buffer containing 20 mM HEPES (pH = 7.5), 10 mM KCl, 250 mM sucrose, 5 mM MgCl 2 , 1 mM EGTA, 1x phosphatase inhibitor cocktail (Sigma, P0044) and 1x protease inhibitor cocktail (Sigma, P8340), and lysed by addition of digitonin (Sigma, D141), 200 g/ml final concentration at 4 C for 10 min

And then, to the extent that we can optimize everything around those factors, whether it be behavior or lifestyle, the current medications theyre on, we also then evaluate that patient if theyre suitable for any type of anti-obesity medication, he said

Together, we can ensure the best possible results

The eligibility of retrieved articles was assessed independently and disagreements were resolved by discussion between the two authors