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Since most studies have investigated the impact of glucagon at doses of 1 mg in humans, resulting in hugely supraphysiological plasma concentrations, many of the published effects of glucagon in humans may be owing to sympathetic nervous system activation [72] and this may also include brown adipose tissue activation

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The sections were washed in a phosphate-buffered saline (PBS) buffer and incubated in a 3, 3-diaminobenzidine (DAB) solution for 3 min