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LCMS analysis was carried out by the URMC Metabolomics Resource

Sertraline was shown to impair both the ligand-induced acrosome reaction and spermatozoa entry into viscous media and it almost completely inhibited the sperm-specific Ca 2+ -permeable ion channel (CatSper), with a significant inhibitory effect on prostaglandin-induced Ca 2+ influx (Figure 1)

Bioactivity was determined using an IGF-1-responsive firefly luciferase reporter assay

Conversely, FOXO1 normally inhibits expression of the GCK gene that encodes glucokinase

The treatment requires multiple sessions (typically 510), costs between 40,000 and 1,20,000 for optimal results, and demands ongoing maintenance every 23 months

10.1016/j.bpc.2020.106327 116 MacKenzieC.GoerkeT.BueckingM.HeidemannM.LeichtleA.RingbeckB.et al (2023)