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Studies have shown that changes in gut microbiota composition can influence the brain via the vagus nerve, production of neurotransmitters such as serotonin, and systemic inflammation, contributing to both gastrointestinal symptoms and psychological comorbidities like anxiety and depression (Ullah et al., 2023)

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Techniques such as molecular docking, inverse virtual screening, and structureactivity relationship studies are integral tools for identifying and refining XO inhibitors with enhanced specificity and potency

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