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Although glutamate is the major downstream product of glutamine, glutamate supplementation during glutamine deprivation cannot rescue the impaired cell growth or mitochondrial respiration 16,43,44,45 , indicating that mitochondrial GLS-catalyzed cleavage of the gamma-nitrogen of glutamine is essential for glutaminolysis

ETD was employed to obtain single amino acid resolution HDX with lower standard deviations than found using a subtractive analysis approach

Applications: This combination may enhance the outcomes of patients undergoing recovery from muscle wasting, surgery, or severe injuries

This result suggests that the cAMP signaling is the main pathway, while the glucose-induced signaling pathway serves as the amplification pathway in GLUTag cells

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Gao X et al