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Ideally, women with a normal pre-pregnancy BMI (19.8-24.9) should gain between 25 and 35 pounds during pregnancy

Notably, reactive astrocytes aberrantly produce GABA from putrescine via MAO-B, releasing it through the BEST1 channel 58,59

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All 12 patients scored a 5/5 on the Global Response Assessment, so this therapy was considered successful

Following 24, 48, 72, or 96 h after seeding, the medium was supplemented with 10 L of CCK-8 reagent (Beyotime) and the cells were incubated at 37 C for 2 h