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The intermediate form of the enzyme is further reduced by the formation of an intra-molecular disulfide bond between the two active site cysteines, leaving the enzyme in an oxidized form, from which the vicinal thiols are regenerated by the FAD(H)-intermediated hydride transfer from NAD(P)H

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Free Radic Biol Med 208:672683 Niu B, Liao K, Zhou Y et al (2021) Application of glutathione depletion in cancer therapy: enhanced ROS-based therapy, ferroptosis, and chemotherapy