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Choline's deficiency threshold is surprisingly low

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Thus, although informative, BiFC studies at the cytosolic face of the ER seem problematic, as previously shown by the analyses of proteins involved in ER-plasma membrane tethering in plant cells (Tao et al., Overexpression of polytopic membrane proteins often results in clustering at the ER, especially when enforced by dimerization via split YFP reconstitution, which we exploited for GPT1 in Baune et al

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Bax monomers form dimer units in the membrane that further self-assemble into multiple oligomeric species