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HEK293 cells were transiently transfected with 21 g of GLP-1R DNA using the FuGENE TM transfection reagent (Roche Applied Science), harvested 24 h after transfection, and used directly in functional experiments or plasma membrane preparations as described previously (12)

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Metabolism (2020) 109:154272

demonstrated GLP-1 RAs broadly suppress perception of all five basic taste modalities, potentially mediated through brainstem GLP-1Rs and vagally mediated pathways 44

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Laboratory assessment of B vitamin levels (serum B12, folate) every 12 weeks provides objective data for fine-tuning dosage