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GLP-1 secretion For secretion experiments cells were plated in 24-well culture plates and allowed to reach 60 to 80% confluency

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The pooled sample was prepared by taking an aliqout (10 l) of each extract, separately for lipidomic and polar metabolite methods, then pooling them, and aliquoting the pool into separate vials

Traditional insulin therapy, while highly effective for glycemic control, typically causes weight gain that can worsen insulin resistance and complicate long-term management