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The manipulation included disrupting the precursor degradation pathways ( tnaA and sdaA ), eliminating l-glutathione degradation ( ggt and pepT ), and manipulating the intracellular ATP level (disruption of glgB )
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The fluorescence intensity of the iNAP1 channel is represented in pseudocolor
For rd1 , the calibration set consists of the [GSH] and [GSSG] measurements at PN11, PN12 and PN13, and the validation set contains the data at PN15, PN17 and PN28