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Electrophoresis was performed with 25 mM Tris, 192 mM Glycine, 0,1% SDS running buffer for about 1 h (130 V)

A few hours later I actually received a phone call as a follow up to the email and answered all of my questions

Viral triggers and reactivation As in other chronic diseases, ME/CFS involves an asymptomatic predisposition stage, then a prodromal stage, followed by symptomatic disease (59)

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