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After brain injury, several pro-inflammatory cytokines are upregulated: interleukin-1 beta, interleukin-6 (IL-6), and tumor necrosis factor (TNF)

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The supernatants were transferred to autosampler vials and analyzed for heroin, morphine, and morphine-3-D-glucuronide (M3G) using an Acquity UPLC system (Waters, Milford, MA) coupled to a Xevo-TQS triple quadrupole mass spectrometer with an electrospray ionization interface (Waters) based on a method previously described 12

Then Chris McGuire, I was always tight with Chris