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Aliquots of 10 L of samples were injected into the Acquity UPLC BEH C18 2.1 mm 100 mm, 1.7-m column (Waters Corporation, Wexford, Ireland), fitted with a C18 precolumn (Waters Corporation, Wexford, Ireland)

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However, if our goal is to significantly alter the course of the opioid crisis, a monotherapy trial is equally as important

doi: 10.1097/GIM.0b013e3181614289 57 SchoonemanM

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Continued use of these models, including newer ones like gut organoids with human microbiota or brain-gut chip systems, will allow dissection of how specific microbes or metabolites act on specific brain cell types