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Increased levels of H 2 O 2 can form extremely reactive hydroxyl radicals through Fenton reactions with free iron present in the cytoplasm, which has downstream negative effects on intracellular calcium levels, protein synthesis, glycosylation, and redox status (53)

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A linear gradient of 0.1% TFA in water (Solvent A) and 0.1% TFA in acetonitrile (ACN) (Solvent B) was used, starting at 99% A with 1% B and increasing per minute at a flow rate of 1 mL/min and detection at 215 nm