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Cell culture and transfection HEK293T and NIH3T3 cells were cultured in DMEM (Sigma-Aldrich) containing 10% FBS and calf serum, respectively, supplemented with penicillin (100 g/ml) and streptomycin (100 g/ml) at 37C with 5% CO 2

The true long-term prognosis of FM patients still needs to be determined in future follow-up studies, and the treatment regimen for these patients should be determined by clinical trials

Por este efecto, es muy importante individualizar segn riesgo-beneficio

From a BMI standpoint, no the thresholds are the same