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2005;42 Suppl(1):S1007 Durand F, Valla D

After denaturing in a boiling water bath, the proteins were separated by SDSpolyacrylamide gel electrophoresis (SDSPAGE), transferred to a PVDF membrane, blocked with 5% milk in TBST for 30 min at room temperature, and then incubated with primary antibody at 4 C overnight with shaking

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[97] [98] A machine learning algorithm has been proposed that discovers subtle patterns in individual history of medical encounters to reliably estimate the risk of a future IPF diagnosis, up to four years before current medical practice