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To assess survival rates, serial dilutions were prepared in Todd Hewitt-Yeast Extract (THYE

doi:10.1111/dom.13979 Frias JP, Davies MJ, Rosenstock J, et al

After reconstitution, aliquot to minimize repeated freeze-thaw cycles

Vitamin E, in particular, is essential for maintaining the integrity of the PUFAs in the sperm membrane, which are highly susceptible to oxidative attack

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