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Reversed-phase HPLC establishes purity and mass spectrometry confirms identity near 1257 Da

Structurally, retatrutide is a 39-amino acid peptide conjugated to a C20 fatty diacid moiety, which confers an extended pharmacokinetic half-life suitable for once-weekly dosing in clinical research settings

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HGH (Research-Grade Human Growth Hormone) is a recombinant peptide supplied in lyophilised vials in 10 IU and 15 IU strengths

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For colony-forming assays, cells were treated with 1.25 M RSL3 overnight, then trypsinized single-cells, diluted 1:300 and seeded into six-well plates