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By using affinity chromatography, one can separate proteins that bind to a certain fragment from proteins that do not bind that specific fragment.Because this technique of purification relies on the biological properties of the protein needed, it is a useful technique and proteins can be filtered multiple folds in a single step

Glutathione synthesis occurs in two ATP-dependent steps catalyzed by the enzymes gamma-glutamylcysteine ligase (GCL, the rate-limiting step) and glutathione synthetase [2]

It was against this background, that prior to leaving Geneva, in the summer of 1962, Jerne sketched out a potential experiment to prove his thinking about the relationship between the antigen and the antibody

doi: 10.1093/jxb/eru109 64

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