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The other is glutamate incorporation into glutathione catalyzed by gamma-glutamylcysteine ligase (GCL), a rate limiting enzyme of glutathione synthesis (Sayin et al

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To ensure consistent peptide concentrations across samples, the digested peptides were quantified using the Pierce Quantitative Colorimetric Peptide Assay (#23275, Thermo Fisher Scientific), a modified BCA assay

Discard any unused reconstituted solution after this period

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NOV-002, a mimetic of glutathione disulfide