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Antibody reactions were detected using the ECL Western blotting detection reagent (Amersham) according to the manufacturer's recommendations

The buffer was pH adjusted to approximately 7.5 and contained 0.1% BSA (Merck KGaA), 5% Dextran T-70 (Dextran Products Limited), 3.5 mmol/L glucose, and 5 mmol/L pyruvate, 5 mmol/L fumarate, and 5 mmol/L glutamate

Why FODMAPs Hit Harder on GLP-1s Delayed stomach emptying means FODMAP-rich foods stay in contact with bacteria longer