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Using Botox as an example, from a clinical perspective, when you inject into a treated upper face muscle: Uptake into the nerve terminal occurs over minutes to hours, and full synaptic blockade may take days Onset of any visible effect is typically within 3-7 days, with the full visible result becoming noticeable at around 10-14 days General treatment duration in upper face indications is typically around 3-4 months, or 12-16 weeks