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The differentiation protocol utilized was developed and optimized from the published literatures 18,19,20,21 , specifically chosen for its capacity to generate iHLOs with high CYP enzyme activity, particularly CYP3A4, which is crucial for drug metabolism and toxicology evaluations

Allow the resin to settle down and the storage buffer to drain from the column

Mechanistically, the blockade of CCL5 increases the production of HGF from reparative macrophages via the CC motif chemokine receptor CCR1- and CCR5mediated forkhead box protein O3 (FOXO3) pathways

2026 Mar 11;148(9):10225-10240

PLoS ONE 8 , e79167e79167 (2013)

Then, aliquot this stock solution into smaller, labeled microcentrifuge tubes