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doi: 10.1016/j.semcdb.2014.02.012 69 Martino-AndradeAJChahoudI

Your choice of binding buffer will influence the binding capacity of the beads , so if you are capturing substantially less than 8 mg of protein per mL of resin, and your protein isnt really big, then it is worth considering if your binding buffer is limiting the capture efficiency of the beads

To overcome this, samples are typically tested at a large dilution to reduce matrix interference and ensure reliable recovery and detection in compliance with USP

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Eccles JC, Llins R, Sasaki K