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Protein extraction and western blot analysis Cells or tumor tissues were lysed using RIPA lysis buffer (P0013B, Beyotime) supplemented with 1 mM PMSF, and the supernatant was collected after centrifugation at 14,000 g for 5 min

Garvey WT, Blher M, Osorto Contreras CK, Davies MJ, Lehmann EW, Pietilinen KH, et al

Taq PCR was performed to amplify the Glp1r cDNA using the following intron-spanning primers, as described by Xu et al

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