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PLoS One 7 e50454 (2012)

It will be interesting to identify the regulatory mechanisms underlying molecular and biochemical responses of different classes of GSTs

Nuclear pellets were resuspended with IP buffer (150 mM NaCl, 50 mM Tris pH 7.5, 1 mM EDTA, 1% Triton), incubated on ice with vigorous regular vortex and sonicated (2x 30 seconds on/off, Picoruptor, Diagenode)

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The digestive system breaks them down before meaningful amounts reach circulation