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Protein was subsequently collected by centrifugation (14,000 g at 4C for 20 minutes) and the supernatant was transferred to an ultrafiltration membrane spin column and centrifuged at 12,500 g for 5 minutes

& Other Lipid Mediat

Hopkins was at the height of a remarkably productive career

Angewandte Chemie

After the polymerase chain reaction (PCR), its products were determined by 1% agarose gel electrophoresis, the target band was cut and purified, and the recovered products were used for Sanger sequencing

Studies have shown that natural products such as polyphenols, terpenes, anthraquinones, and sulforaphane can activate the hepatocyte antioxidant defense system with Nrf2 as the core player, reduce oxidative stress damage, and protect the liver